徐瑶,陈慧敏,陶栓,张鹤,戴燕萍,方野微,陈鲁燕,梁伟

• 1:宁波大学附属第一医院
• 2:宁波大学医学部
• 3:蚌埠医科大学检验医学院

摘要(Abstract)：

目的 分析新型冠状病毒（severe actue respiratory syndrome coronavirus 2, SARS-CoV-2）在高流行背景下核酸初检阳性病例分别采用核酸和抗原检测复核后的结果变化特征。方法 收集2022年12月11―15日宁波大学附属第一医院发热门诊SARS-CoV-2核酸初检阳性[ORF1ab或N基因循环数阈值（Ct值）<40]的患者样本共624份，其中，562份初检双靶（ORF1ab和N基因）阳性，62份初检单靶（ORF1ab或N基因）阳性。562份初检双靶阳性样本中，322份进行核酸复检，240份进行抗原复检。对62例初检单靶阳性样本直接进行核酸复检。比较初检与复检靶基因（ORF1ab和N）的Ct值和阳性率。结果 初检双靶阳性样本经核酸复检后，复检阳性组（双靶或单靶阳性）与复检阴性组初检时的靶基因Ct值[ORF1ab基因：（33.87±3.15）比（37.06±1.81）;N基因：（33.64±3.22）比（36.87±1.95）]差异均有统计学意义（t值分别为-8.26和-8.13,P均<0.01）。复检ORF1ab[(33.73±3.14)比（36.35±2.65）]和N基因[（33.29±3.27）比（35.94±2.71）]的Ct值均显著升高（t值分别为-19.756和-19.477,P均<0.01）。抗原复检结果：236例（236/240, 98.3%）为抗原阳性，高于核酸复检阳性率（249/322, 77.33%）; 4例（4/240,1.67%）为抗原阴性，其初检时的靶基因Ct值均>34。初检单靶阳性样本经核酸复检其Ct值与初检差异无统计学意义。结论 SARS-CoV-2核酸初检阳性样本复检后ORF1ab或N基因的Ct值升高，假阴性的可能性增加。而抗原复检阳性率高于核酸，提示抗原检测可作为初检双靶阳性样本快速复核的有效手段。

关键词(KeyWords)： 新型冠状病毒;核酸检测;抗原检测;Ct值;结果复核

Abstract:

Keywords:

基金项目(Foundation): 宁波市科技计划项目（2022Z220,2023J020）;; 中国人口福利基金会医学创新课题（SLB-6-20230912-351）;; 宁波大学附属第一医院院级课题（新型冠状病毒感染疾病科技攻关专项）（XGZ2303）

作者(Author): 徐瑶,陈慧敏,陶栓,张鹤,戴燕萍,方野微,陈鲁燕,梁伟

DOI: 10.16505/j.2095-0136.2024.0103

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